中国水稻科学Issue(6)：633-638,6.DOI:10.3969/j.issn.1001-7216.2013.06.010
酵母双杂交筛选灰飞虱体内水稻黑条矮缩病毒 p10的互作蛋白
Screening of RBSDV p10 Interacting Proteins in Small Brown Planthoppers by Yeast Two Hybrid System
摘要
Abstract
To reveal protein factors in Laodelphax striatellus Fallen(the small brown planthopper,SBPH)that related to Rice black-streaked dwarf virus (RBSDV)transmission,the outer capsid protein p10 encoded by RBSDV S10 was selected as bait to screen the SBPH cDNA library.RBSDV p10 gene was inserted to pGBKT7 vector and the bait plasmid pGBKT7-p10 was constructed.The result of auto-activation test showed that p10 could not autonomously activate the expression of reporter genes in yeast and had no toxicity to yeast cell.To obtain the interacting proteins with p10,the pGADT7-cDNA library plasmids were transformed to the yeast AH109 which contains pGBKT7-p10. Three hundred and twenty-six positive clones were acquired.The sequencing results show that these positive clones encode 14 proteins,including Actin 1,GAPDH and RACK.These interacting proteins were involved in several processes such as endocytosis,exocytosis and membrane fusion.The interactions between p10 and these proteins may be related to the viral circulation and proliferation in insect vector.关键词
水稻黑条矮缩病毒/外壳蛋白/互作蛋白/酵母双杂交Key words
Rice black-streaked dwarf virus/capsid protein/interacting protein/yeast two hybrid分类
农业科技引用本文复制引用
徐秋芳,陈晴晴,张金凤,李硕,倪海平,周益军..酵母双杂交筛选灰飞虱体内水稻黑条矮缩病毒 p10的互作蛋白[J].中国水稻科学,2013,(6):633-638,6.基金项目
国家自然科学基金资助项目(31000841) （31000841）
公益性行业(农业)科研专项(201003031) （农业）
江苏省农业科学技术自主创新资金项目[CX(13)5019 （13）
