中国水稻科学Issue(1)：32-40,9.DOI:10.3969/j.issn.1001-7216.2014.01.005
干旱驯化后节水抗旱稻苗期不同发育时间DNA甲基化模式变化分析
Changes in DNA Methylation Pattern in a Water-saving and Drought-resistance Rice Variety at Three-leaf and Four-leaf stages after Drought Domestication
摘要
Abstract
A water-saving and drought-resistance rice Huhan 3 was grown under drought stress for six successive generations after transplanting in the field.The variations in DNA methylation pattern between the original generation (G0 )and the sixth generation (G6 )were analyzed by methylation sensitive amplification polymorphism (MSAP) method.The results revealed that the methylated loci accounted for about 34% of the total.Among these,about 84%were full-methylated loci and about 1 6% were hemi-methylated ones.The DNA methylation level decreased from the three-leaf stage to the four-leaf stage in Huhan 3.Differentially methylated loci (DML)between generations or/and between different developmental stages accounted for 4.0% of the total,most of which were only related to plant development(5 7��9%).Compared to G0 ,the DNA methylation pattern of G6 changed after drought domestication,at three-leaf stage,de-methylated events occupying 59.1%,while at four-leaf stage,re-methylated events occupied 47.9%.Genome-wide alternations of DNA methylation were observed along with rice growth and development,and DML mainly occurred on the gene′s promoter and exon region.The genes with DML were involved in a wide range of functions and participated in many important biological processes.关键词
干旱驯化/节水抗旱稻/DNA甲基化/甲基化敏感扩增多态性Key words
drought domestication/water-saving and drought-resistance rice (WDR)/DNA methylation/methylation-sensitive amplification polymorphism (MSAP)分类
农业科技引用本文复制引用
郑小国,陈亮,楼巧君,夏辉,李明寿,罗利军..干旱驯化后节水抗旱稻苗期不同发育时间DNA甲基化模式变化分析[J].中国水稻科学,2014,(1):32-40,9.基金项目
国家863计划资助项目(2012AA101102) （2012AA101102）
上海市科技兴农重点攻关项目[沪农科攻字(2010)第1-1号] （2010）
高层次科技人才培引工程(2010C1120) （2010C1120）
