中国水稻科学Issue(2)：125-131,7.DOI:10.3969/j.issn.1001-7216.2014.02.003
水稻千粒重 QTL qTGW1.1的验证
Validation of qTGW1 .1,a Quantitative Trait Locus for 1000-grain Weight in Rice (Oryza sativa L.)
摘要
Abstract
This study was conducted to validate a quantitative trait locus (QTL)for 1000-grain weight (TGW)which was previously mapped in a 3.7 Mb region on the long arm of rice chromosome 1 ,and to locate this QTL more precisely.A plant carrying a heterozygous segment extending from RM1 1448 to RM1 1 6 1 5 was selected from the Zhenshan 97 3/Milyang 46 BC2 F5 population.The resulting BC2 F6 and BC2 F7 populations were assayed with SSR markers.Three BC2 F7 plants were identified,carrying heterozygous segments covering the intervals RM1 1448 -RM1 1 522,RM1 1448 - RM1 1 549 and RM1232 - RM1 1 6 1 5 , respectively.Non-recombinant homozygotes were identified from the BC2 F8 populations,from which three sets of near isogenic lines (NILs)in the BC2 F8 ∶9 generation were produced,consisting of two parental genotypes in the intervals RM1 1448-RM1 1 522,RM1 1448-RM1 1 549 and RM1232-RM1 1 6 1 5 ,respectively.Field trials were conducted to measure heading date (HD),TGW,and grain shape traits including grain length and width.Two-way analysis of variance was performed to test the phenotypic differences between two genotypic groups in each NIL set using SAS program.No significant variations were observed on TGW and grain shape in the NIL which was heterogeneous in the interval RM1 1448 -RM1 1 522,whereas significant genotypic effects were detected in the remaining two NILs in which the enhancing alleles were all derived from Zhenshan 97.For HD,no significant effects were detected in the three NILs.Based on comparison among genomic locations of the three heterogeneous regions,qTGW1 .1 was delimited to a 688.8 kb region flanked by RM1 1 522 and RM1 1 554.关键词
水稻(Oryza sativa L.)/数量性状座位/微效作用/千粒重/验证Key words
rice (Oryza sativa L.)/quantitative trait locus/minor effect/1000-grain weight/validation分类
生物科学引用本文复制引用
张宏伟,朱玉君,陈玉宇,陈俊宇,黄得润,庄杰云..水稻千粒重 QTL qTGW1.1的验证[J].中国水稻科学,2014,(2):125-131,7.基金项目
国家863计划资助项目(2012AA101102) （2012AA101102）
国家超级稻育种专项(201301) （201301）
中央级公益性科研院所基本业务费专项(2012RG002-3) （2012RG002-3）
