中国水稻科学Issue(2)：141-147,7.DOI:10.3969/j.issn.1001-7216.2014.02.005
稻瘟病抗病基因Pi-kh功能标记的开发及江苏粳稻品种中Pi-kh的变异
Development of a Functional Marker for Rice Blast Resistance Gene Pi-kh and Natural Variation at Pi-kh Locus in japonica Rice in Jiangsu Province,China
摘要
Abstract
Pi-kh is a vital non race-specific resistance gene for rice blast.In this study,one InDel marker,FM143 was developed based on the 143 bp insertion between the susceptible and resistant Pi-kh alleles.Previous studies showed that Tetep carried resistant Pi-kh allele and had the none-insertion genotype for PCR by FM143.F2 population,derived from Tetep and Ning 9108,were detected by marker FM143 and the result indicated that FM143 could effectively identify Pi-kh/Pi-kh ,Pi-kh/pi-kh and pi-kh/pi-kh genotypes with the expected segregation ratio 1∶2∶1.Sixty-five japonica varieties widely grown in Jiangsu Province over years and sixty-four japonica lines were genotyped by FM143. Among them,19 japonica varieties and 22 japonica lines showed none-insertion genotype.Further sequencing at the target Pi-kh locus for these none-insertion genotype showed that there was one highly conserved single-base mutation (G→C)at 102 bp location compared with the whole target gene sequence in Tetep and public Pi-kh GeneBank leadingPi-khJSJ.关键词
水稻/稻瘟病/Pi-kh/功能标记/Pi-kh JSJKey words
rice/rice blast/Pi-kh/functional marker/Pi-kh JSJ分类
农业科技引用本文复制引用
王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功..稻瘟病抗病基因Pi-kh功能标记的开发及江苏粳稻品种中Pi-kh的变异[J].中国水稻科学,2014,(2):141-147,7.基金项目
国家科技支撑计划重大项目(2011BAD16B03) （2011BAD16B03）
国家自然科学基金资助项目(31101131) （31101131）
江苏省农业自主创新资金资助项目[CX(12)1003] （12）
江苏省科技支撑计划资助项目(BE2012309) （BE2012309）
江苏省自然科学基金资助项目(BK2011666) （BK2011666）
