中国水稻科学Issue(2)：155-166,12.DOI:10.3969/j.issn.1001-7216.2014.02.007
抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响
Effect of High Temperature Stress on Physiological Characteristics of Anther, Pollen and Stigma of Rice During Heading-flowering Stage
摘要
Abstract
Six rice recombinant inbred lines with different heat tolerance and their parents,heat tolerant line 996 and heat sensitive line 4628 were treated with high temperature (9:00 - 1 7:00,37℃;1 7:00 - 9:00,30℃)and optimal temperature (9:00 - 1 7:00,30℃;1 7:00 - 9:00,25 ℃)in growth chambers.The effect of high temperature on antioxidant enzyme activities,membrane permeability,souble sugar content,heat stable protein content of anther, pollen grain and stigma viability were studied.The results showed the superoxide dismutase(SOD),catalase (CAT), peroxidase (POD)activities in anther increased obviously at early stage under high temperature stress,then decreased rapidly,the antioxidant enzyme activity of anther of heat tolerant lines was significantly higher than that of heat sensitive lines.Compared with heat sensitive lines,higher heat stable protein content and souble sugar content,lower malondial dehyde (MDA)content and relative conductivity were found in anther of heat tolerant lines.The anther dehiscence coefficient,pollen grains number on the stigma,pollen viability and stigma viability of heat tolerant lines were significantly higher than those of heat sensitive lines.It showed that hybrids might inherit the heat tolerance from the parent 996.The antioxidant enzyme activity,membrane permeability,heat stable protein content of anther and pollen viability can be used as heat resistance identification indexes in rice.关键词
水稻重组自交系/抽穗开花期/高温胁迫/花器官/生理特性Key words
rice/recombinant inbred lines/heading stage/high temperature stress/flower organ/physiological charac-teristics分类
农业科技引用本文复制引用
张桂莲,张顺堂,肖浪涛,唐文帮,肖应辉,陈立云..抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响[J].中国水稻科学,2014,(2):155-166,12.基金项目
国家自然科学基金资助项目(30900874) （30900874）
湖南省自然科学基金资助项目(11JJ3026) （11JJ3026）
湖南省科技计划博士后专项(2012RS4037) （2012RS4037）
湖南省教育厅青年基金项目(YB2009B023) （YB2009B023）
作物学开放基金资助项目(ZWKF201301) （ZWKF201301）
