中国生物防治学报Issue(4)：521-530,10.
产酶溶杆菌OH11双精氨酸转运系统关键基因tatC的功能分析
Functional Analysis of Twin-arginine Translocation Gene tatC in Lysobacter enzymogenes Strain OH11
摘要
Abstract
Twin-arginine translocation gene (tatC) of Lysobacter enzymogenes strain OH11 was knocked out by double-cross homologue recombination. Functions of tatC were investigated by comparisons of phenotypes and phenotype-associated gene expressions between wild type and tatC mutant, more attentions were paid on the relationship between tatC and HSAF, an antifungal secondary metabolite produced by L. enzymogenes. Compared with wild-type OH11, mutation of tatC significantly increased the production of HSAF and the transcriptional expression level of pks/nrps, which was a key gene responsible for HSAF biosynthesis. It was observed that the normal clavated cell morphology was changed into ellipticity when disruption of tatC in L. enzymogenes. Furthermore, mutation of tatC remarkably impacted the growth rate in nutrient-limiting broth, biofilm formation and gliding motility. However,MtatC mutation of tatC did not impact the growth rate in nutrient−rich broth, four−type lytic enzyme production, and inhibitory activities against Pythium apanidermatum, Rhizoctonia solani and Fusarium graminearum.关键词
产酶溶杆菌/基因tatC/敲除/功能分析Key words
Lysobacter enzymogenes/tatC gene/knock out/functional analysis分类
农业科技引用本文复制引用
徐菲菲,钱国良,汪郁兰,张娟,范加勤,胡白石,刘凤权..产酶溶杆菌OH11双精氨酸转运系统关键基因tatC的功能分析[J].中国生物防治学报,2013,(4):521-530,10.基金项目
公益性行业（农业）科研专项（200903052；201003004；201203034）；国家“863”计划项目（2011AA10A205）；国家自然科学基金（31101478）；教育部博士点基金（20100097120013）；现代农业产业技术体系（nycytx-29-09）；国家“948”项目 （）
