中国生物防治学报Issue(4)：569-578,10.
解淀粉芽孢杆菌HF-01发酵条件优化
Optimizing Fermentation Condition for Bacillus amyloliquefaciens HF-01
摘要
Abstract
In order to investigate best condition for liquid fermentation of Bacillus amyloliquefaciens HF-01 and increase the antagonistic substance productivity of strain HF−01, the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions. The optimum medium was 3.0%glucose, 0.5%yeast extract powder, 0.3%NaCl and 0.1%MgSO4. The optimal fermentation conditions were initial pH 6.0-6.5, temperature 28℃, fermentation time 48 h, medium capacity 90 mL/250 mL, inoculation rate 6%, and rotate speed 130 r·min−1. Inhibitory activity of strain HF−01 fermentation solution increased 37.3% under the optimum fermentation medium and culture conditions. When optimized filtrate was treated on citrus fruits under 25 ℃ for 4 days, the disease incidence and lesion diameter were 31.7%and 25.9 mm respectively, significantly lower than unoptimized filtrate with 56.7% disease incidence and 48.1 mm lesion diameter.关键词
解淀粉芽孢杆菌/发酵条件优化/单因子试验/正交试验/柑桔绿霉病菌Key words
Bacillus amyloliquefaciens/fermentation condition optimization/single factor experiment/orthogonal experiment/Penicillium digitatum分类
农业科技引用本文复制引用
洪鹏,安国栋,胡美英,耿鹏,桑松..解淀粉芽孢杆菌HF-01发酵条件优化[J].中国生物防治学报,2013,(4):569-578,10.基金项目
国家自然科学基金项目 （）
