中国生物防治学报Issue(4)：661-666,6.
种子处理诱导大豆抗胞囊线虫病的生防细菌筛选与鉴定
Screening and Identification of Bacterium to Induce Resistance of Soybean against Heterodera glycines
摘要
Abstract
Soybean plants are able to develop resistance against Heterodera glycines when coated with Bacillus simplex. Three bio-control bacteria were selected after a two-year field experiment. Among them, strain Sneb545 was proven effective by pot culture in greenhouse. In order to elucidate the modes of action, the split−root experiment was designed to investigate Sneb545’s ability to induce soybean systemic resistance against H. glycines. The results showed that soybean seeds treated with strain Sneb545 were able to produce seedlings that had notable resistance to first generation cyst nematodes. Number of H. glycines cyst in soybean roots decreased by 72.63%comparing with control, the ratio of H. glycines in adjacent soil to soybean root decreased by 70.63%. Split-root experiment concluded that after inoculating Sneb545 in the challenging roots, nematodes penetration in responding roots decreased by 51.27%, number of cyst in soil decreased by 65.82%. Sneb545 was identified as B. simplex based on morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis.关键词
诱导抗性/大豆胞囊线虫/简单芽孢杆菌/发酵液Key words
induced resistance/Heterodera glycines/Bacillus simplex/fermentation broth分类
农业科技引用本文复制引用
项鹏,陈立杰,朱晓峰,王媛媛,段玉玺..种子处理诱导大豆抗胞囊线虫病的生防细菌筛选与鉴定[J].中国生物防治学报,2013,(4):661-666,6.基金项目
公益性行业（农业）科研专项（200903040-03）；国家现代农业产业技术体系 （）
