中国生物防治学报Issue(1)：73-78,6.
Bt菌株FB的生物学特性及其cry、vip基因型鉴定
Biological Characteristics of Bacillus thuringiensis FB Strain and Identification of Its cry and vip-type Genes
摘要
Abstract
Bacillus thuringiensis FB strain was high toxicity against larvae of Plutella xylostella (L.). FB strain was characterized with scanning electron microscope, large plasmids electrophoresis, SDS-PAGE and growth characteristics observation. Five full length toxin genes, cry1Ia, cry1Ea, cry1Ab, cry2Ab and vip3Aa, were cloned. Furthermore, a half-length fragment of cry8 gene was found by whole-genome sequencing, this is the first time that cry1 and cry8-type genes existed in the same strain. The highest identity of the deduced amino acid sequences with known B. thuringiensis toxins were 99%, 98%, 99%, 100%, 99%respectively, but deduced amino acid sequence of this Cry8 fragment was 63%similarity with known Cry8 proteins. Bioassay results showed that Cry1Ab, Cry1Ia, Cry1Ea were high toxicity against larvae of P. xylostella.关键词
苏云金芽胞杆菌/菌株FB/表达/杀虫活性/小菜蛾Key words
Bacillus thuringiensis/FB strain/expression/insecticidal activity/Plutella xylostella分类
农业科技引用本文复制引用
王品舒,刘晓垒,席景会,彭琦,束长龙,张杰,宋福平..Bt菌株FB的生物学特性及其cry、vip基因型鉴定[J].中国生物防治学报,2014,(1):73-78,6.基金项目
国家自然基金项目（31270111）；国家“863”计划项目 （）
