中国生物防治学报Issue(1)：134-142,9.
溶杆菌SNNU513基因gfp标记及在玉米根部定殖
Colonization of gfp-tagged Lysobacter sp. SNNU513 on Maize’s Root
摘要
Abstract
Lysobacter sp. SNNU513 isolated from Radix polygalae rhizospher could inhibit pathogenic fungus. In this study, the preparation of the efficient competent cells were screened out and pGLO plasmid was introduced into the competent cells by electroporation and transformation. The results showed that the recombinant strain SNNU513-pGLO could efficiently and stably express the green fluorescent protein gene (gfp) when the electric field strength was 20 kV/cm and the electric pulse time was 5 ms. The percent of the green cells was 100%when the strain SNNU513-pGLO was recirculated in the non-selective medium for 20 times. Both strains SNNU513-pGLO and SNNU513 showed the same growth characteristics and the same inhibition of Rhizotonia cerealis in vivo. The gene gfp was transferred into original strain Lysobacter sp. SNNU513 by electroporation successfully. The gfp-tagged strain adhering more tightly to the velamen than the phloem could colonize on the surface and interior of maize’s root.关键词
生防菌/溶杆菌属/绿色荧光蛋白基因gfp/生物学特性/定殖Key words
biocontrol bacteria/Lysobacter/gfp/biological characteristics/colonization分类
农业科技引用本文复制引用
武坤毅,王斐斐,崔浪军,章华伟,白成科..溶杆菌SNNU513基因gfp标记及在玉米根部定殖[J].中国生物防治学报,2014,(1):134-142,9.基金项目
国家自然科学基金（31101481）；浙江省公益性技术应用研究项目 （）
