中国生物防治学报Issue(2)：216-221,6.
枯草芽孢杆菌OKB105产生的surfactin防治烟草花叶病毒病及其机理研究
Effect and Mechanism of Controlling TMV Disease on Tobacco by Surfactin Produced by Bacillus subtilis OKB105
摘要
Abstract
In order to interpret resistance mechanism, controlling effect of surfactin against TMV on tobacco and the expression pattern of defense-related genes were investigated. Liquid culture of Bacillus subtilis OKB105 was precipitated by 6 mol/L HCl. The crude extract of lipopeptides was dissolved by methanol and separated by LH20 chromatographic column. The crude extract detected surfactin only by MALDI-TOF-MS and hemolytic activity analysis. Tobacco was infected with TMV after pretreated by 0.01 g/L surfactin, the result showed that surfactin could control TMV efficiently, the controlling effect reached to 70.9%. The expressions of SA-pathway genes PR1, PR3, PR5, and ET-pathway gene ETR1 were obviously increased by real-time PCR test. The results demonstrated that surfactin could elicite ISR (induced systemic resistance) reaction which depend on SA-pathway and ET-pathway.关键词
surfactin/烟草花叶病毒病/防治效果/诱导系统抗性Key words
surfactin/TMV disease/control efficiency/ISR分类
农业科技引用本文复制引用
任鹏举,谢永丽,张岩,伍辉军,高学文..枯草芽孢杆菌OKB105产生的surfactin防治烟草花叶病毒病及其机理研究[J].中国生物防治学报,2014,(2):216-221,6.基金项目
国家自然科学基金（31100056）；高等学校博士学科点（20100097120011）；公益性行业（农业）科研专项（201303015）；国家“863”计划 （）
