中国生物防治学报Issue(2)：239-246,8.
36种中草药提取物杀松材线虫活性研究
Toxicity of Thirty-six Chinese Medicinal Herbs against Bursaphelenchus xylophilus
摘要
Abstract
Toxicity of ethanol extracts of thirty-six traditional Chinese medicinal (TCM) herbs to adult nematodes and eggs of Bursaphelenchus xylophilus were evaluated in this study to find efficient nematicidal compounds. Bioassay results showed that the ethanol extracts of Cortex moutan radicis, Coptis chinensis franch and Radix et rhizomarhei possessed excellent efficacy with 100%mortality of adults, less than 20%egg hatching rate and more than 60%mortality of eggs at the concentration of 10 g/L. The LC50 of ethanol extracts of three TCM herbs were 1.81, 0.2638 and 1.07 g/L respectively against B. xylophilus after treatment of 72 h. Preliminary separation of active ingredients of three TCM herbs using ethyl acetate and water was employed to examine the nematicide activities of the extracts. The active ingredients of C. moutan radicis presented in ethyl acetate extract, while the active ingredients of C. chinensis franch and R. et rhizomarhei presented in water extracts. The LC50 of active ingredients from three TCM herbs were 0.712, 2.370 and 0.067 g/L respectively against B. xylophilus after treatment of 72 h. This research will provide a theoretical basis for the development of novel biorational nematicide.关键词
中药提取物/松材线虫/杀线虫活性Key words
Chinese herb extracts/Bursaphelenchus xylophilus/nematicidal activity分类
农业科技引用本文复制引用
焦宏伟,张璐,赵宝华,刘东..36种中草药提取物杀松材线虫活性研究[J].中国生物防治学报,2014,(2):239-246,8.基金项目
河北师范大学博士基金项目（L2010B10）；河北师范大学校内基金项目 （）
