中国比较医学杂志Issue(3)：78-82,5.DOI:10.3969/j.issn.1671.7856.2014.003.016
心脏组织特异性表达 Neuregulin -2转基因小鼠的建立及心脏功能分析
The establishment of cardiac-specific human Neuregulin-2 transgenic mice and cardiacfunction analysis
摘要
Abstract
Objective To study the effects of NRG2 on cardiac structure and function , we established the cardiac-specific human NRG2 transgenic mice and investigate the effect of NRG2 on cardiac structure and function under pressure overload situation .Methods The transgenic vector was constructed by insertion of the human NRG2 gene under the α-MHC promoter.The transgenic mice were generated by microinjection and were all maintained on a C57BL/6J genetic background .The genotype of transgenic mice was identified by PCR and the expression level of target gene was determined by western blot .Transverse aortic constriction ( TAC) was applied to prepare the pressure overload induced cardiomyopathy mice model .The cardiac structure and function of the transgenic mice were compared and analysized by echocardiographic and pathological observation .Results Transgenic mice with high level of NRG2 in heart tissues were established.The left ventricular wall thickness (LVPWD) was increased, and to 15.6% at 3 months old compared with that of the non transgenic ( NTG) mice.The hypertrophy of left ventricular wall caused by pressure overload was removed due to the expression of NRG2 .Meanwhile, cardiac disarray and fibrosis were increased obviously compared with that of the NTG mice.Conclusion The transgenic expression of NRG2 in heart tissues could shorten the pathological process of hypertrophy, but accelerated the process of heart failure (HF).关键词
NRG2/转基因/压力负荷/心室重构Key words
NRG2/Transgene/Pressure overload/Ventricular remodeling分类
医药卫生引用本文复制引用
路迎冬,鲍丹,刘宁,张旭,马元武,吕丹,张连峰..心脏组织特异性表达 Neuregulin -2转基因小鼠的建立及心脏功能分析[J].中国比较医学杂志,2014,(3):78-82,5.基金项目
国家科技支撑计划课题（2012BA139B02）。 （）
