中国比较医学杂志Issue(4)：13-18,6.DOI:10.3969/j.issn.1671.7856.2014.004.004
长爪沙鼠ApoE基因SNPs的遗传多态性
Evaluation of genetic polymorphisms of ApoE gene in Mongolian gerbils based on single nucleotide polymorphisms
摘要
Abstract
Objective To evaluate the genetic diversity of ApoE gene in Z:ZCLA gerbil closed colony .Methods PCR-SSCP technique was employed to calculate the gene frequency , genotype frequency , heterozygosity , polymorphism information content of three SNPs of ApoE gene , which were reported in our previous work , in a total of 444 ordinary and clean Mongolian gerbils .Results The average allele of 3 SNP site 97, 981 and 1774 was 2.The inheritance pattern was in accordance with Mendel′s law.Expected heterozygosities were 0.063, 0.501, and 0.499, and the average expected heterozygosity of whole group was 0.354.PIC were 0.061, 0375, and 0.374, with an average PIC of 0.270.Conclusion The ApoE gene frequency and genotype distribution in Mongolian gerbils may be to a certain degree associated with the genetic drift caused by long-term closed and selected breeding .关键词
长爪沙鼠/高脂血症/ApoE基因/SNPs/遗传多态性Key words
Mongolian gerbil/Hyperlipidemia/apolipoprotein E (ApoE) gene/Single nucleotide polymorphisms (SNPs)分类
医药卫生引用本文复制引用
刘月环,王志远,杜江涛,余强,余陈欢,吴旧生,应华忠..长爪沙鼠ApoE基因SNPs的遗传多态性[J].中国比较医学杂志,2014,(4):13-18,6.基金项目
浙江省自然科学基金（Y3080126），浙江省医药卫生优秀青年人才基金（2007QN001），浙江省级公益性技术应用研究计划（2011C37096），省医药卫生平台重点研究计划（2011ZDA002），浙江省公共科技服务计划项目（2012F30026）。 （）
