中国比较医学杂志Issue(4)：23-27,5.DOI:10.3969/j.issn.1671.7856.2014.004.006
小鼠α-synuclein 基因内含子1甲基化定量检测方法的建立及不同脑区甲基化水平的比较研究
Establishment of a method to measure the methylation level of mouse α-synuclein intron-1 and comparison of its methylation at different brain regions
摘要
Abstract
Objective To establish a quantitative method to examine the methylation level of CpG island located in the intron 1 of mouse α-synuclein, and analyze the difference of methylation in various brain regions .Methods CpG island in the intron 1 of mouse α-synuclein was identified using an online software MSPprimer .Pyrosequencing assay was designed using PyroMark assay design 2.0.DNA methylation was quantified in mouse hippocampus , cortex, striatum and midbrain using pyrosequencing assay .Results There was a CpG island in the intron 1 of mouse α-synuclein. Pyrosequencing assay measuring methylation level of this CpG island was optimized .It indicated that all of the brain regions were hypomethylated with a methylation rate of 2%or less.Conclusion DNA methylation does not play a major role in the transcription regulation of mouse α-synuclein in the brain .关键词
α-突触核蛋白/DNA甲基化/焦磷酸测序/小鼠/脑/中脑/海马/纹状体/皮质Key words
α-synuclein/DNA methylation/Pyrosequencing/Brain/Cortex/Midbrain/Striatum/Hippocampus/Mouse分类
医药卫生引用本文复制引用
陆璐,张兰,王丹慧,蔡彦宁..小鼠α-synuclein 基因内含子1甲基化定量检测方法的建立及不同脑区甲基化水平的比较研究[J].中国比较医学杂志,2014,(4):23-27,5.基金项目
国家自然科学基金（编号81274120）；北京市自然科学基金（编号7132110）；北京市新世纪百千万人才工程项目（编号008-0014）。 （）
