中国比较医学杂志Issue(4)：51-56,6.DOI:10.3969/j.issn.1671.7856.2014.004.012
高脂血症长爪沙鼠模型的转录组检测和代谢性炎症通路的初步研究
Detection of transcriptome in hyperlipidemia and metabolic/inflammatory pathways in Mongolian gerbils
摘要
Abstract
Objective To understand some key genes and metabolic/inflammatory pathways triggered by high fat/high cholesterol ( HF/HC ) diet-induced hyperlipidemia in Mongolian gerbil .Methods Sixty 90-day-old Mongolian gerbils (including both sexes) were used in this study.The gerbils were divided into normal group (n=30, control diets) and model group (n=30, Hf/HC diets).Hyperlipidemia was induced for 4 weeks.To extract mRNA for transcriptome by RNA-Seq deep sequencing and bioinformatics .Results The results showed that 47 522 de novo gerbil genes (≥100 bp unigene ) were obtained , with a total of 26.9 Mb, and about 82.53% sequence was homologous with the exon in the common genome bases .There were 21 125 differential genes between the hyperlipidemia gerbil and normal gerbil groups , including 16 087 down-regulated and 5038 up-regulated genes .There was a significant trend of downregulation compared with the normal group ( P <0.01 ) .There were eight pathways significantly related to the metabolic inflammation in hyperlipidemia ( P<0.01) , and among the expressed genes of these pathways , 15 immune-and inflammation-related genes were significantly down-regulated ( P<0.01 ) .Conclusions RNA-Seq and bioinformatics techniques can be used as a powerful tool for overall gene-transcription in hyperlipidemic gerbil models .Some key genes and pathways screened in this study may become candidate genes or operating targets in the future research .关键词
长爪沙鼠/高脂血症/二代测序技术/转录组/代谢性炎症通路Key words
Mongolian gerbil/Hyperlipidemia/RNA-Seq/Ttranscriptome/Metabolic/inflammatory pathways分类
医药卫生引用本文复制引用
刘月环,毛栋森,吴旧生,钟宇森,周莎桑,金晓音,柯贤福,应华忠..高脂血症长爪沙鼠模型的转录组检测和代谢性炎症通路的初步研究[J].中国比较医学杂志,2014,(4):51-56,6.基金项目
浙江省自然科学基金（Y3080126），浙江省级公益性技术应用研究计划（2011C37096），省医药卫生平台重点研究计划（2011ZDA002），浙江省医药卫生优秀青年人才基金（2007QN001），浙江省公共科技服务计划项目（2012F30026）。 （）
