中国动物传染病学报Issue(5)：63-67,5.
猪伪狂犬病毒安徽株gE基因的克隆和序列分析
CLONING AND SEQUENCE ANALYSIS OF GE GENE OF PORCINE PSEUDORABIES VIRUS ANHUI STRAINS
摘要
Abstract
Porcine pseudorabies virus (PRV) isolates were obtained recently from infected pigs in Anhui area. The gE gene of the PRV isolates was amplified using a pair of primers based on GenBank database. The resulting PCR products were cloned and sequenced. The amplified fragments were 632 bp. The sequence homology and phylogenetic analyses were performed using DNAStar software. Eight PRV isolates from Anhui shared 96.8%-99.7%nucleotide sequence identity and 97.9%-100.0%amino acids identity among each other while these PRV isolates shared 97.0%-99.7%nucleotide sequence identity and 95.2%-100.0%amino acids identity with other reference strains from GenBank. Phylogenetic analysis indicated that PRV isolates from Anhui had a closer relationship with other domestic isolates than foreign reference strains.关键词
猪伪狂犬病病毒/gE基因/克隆/序列分析Key words
Porcine pseudorabies virus/gE gene/phylogenetic analysis/sequence analysis分类
农业科技引用本文复制引用
占松鹤,殷冬冬,姜安安,郭浩,朱良强,范红结,王桂军..猪伪狂犬病毒安徽株gE基因的克隆和序列分析[J].中国动物传染病学报,2013,(5):63-67,5.基金项目
安徽省教育厅自然科学基金项目（KJ2012A124）；安徽农业大学大学生创新基金项目 （）
