中国动物传染病学报Issue(6)：26-32,7.
副溶血弧菌SHJLA株的分离鉴定及致病性分析
ISOLATION, IDENTIFICATION AND PATHOGENICITY OF VIBRIO PARAHAEMOLYTICUS STRAIN SHJLA
摘要
Abstract
VibrioParahaemolyticus is a common food-borne pathogen in marine environment. In the present study, a bacterial strain was isolated from a shrimp sample and identified it as Vibrio parahaemolyticus strain SHJLA by its morphological, physiological and biochemical characteristics. The SHJLA strain formed blue-green colonies on TCBS and aubergine colonies on vibrio chromogenic medium. Its physiological and biochemical characteristics also met typical features of V.parahaemolyticus. The SHJLA strain posseessed specific genes (tlh、toxR、groEL) of V.parahaemolyticus. Sequence analysis of gyrB gene showed that SHJLA strain shared 98%-100%identity to published V. parahaemolyticus data. In addition, important virulence related genes (tdh and T3SS2) of V. parahaemolyticus were also found in SHJLA strain. The SHJLA strain showedβhemolytic reaction and was KP positive. When mice were intraperitioneally injected with SHJLA strain, it had high virulence as measured by the median lethal dose (LD50) of 4.8×108 cfu/mL.关键词
副溶血弧菌/菌落特征/生理生化特征/毒力基因/神奈川实验/小鼠半数致死量Key words
Vibrioparahaemolyticus/colony/biochemical reaction/virulence/Kanagawa test/median lethal dose分类
农业科技引用本文复制引用
蒋蔚,李欣彤,何再平,刘迎春,陈永军,王权,刘永杰..副溶血弧菌SHJLA株的分离鉴定及致病性分析[J].中国动物传染病学报,2013,(6):26-32,7.基金项目
国家科技支撑计划（2012BAK08B07）；上海市科学技术委员会科研计划项目 （）
