中国动物传染病学报Issue(1)：44-49,6.
水貂绿脓杆菌的分离与鉴定
ISOLATION AND IDENTIFICATION OF PSEUDOMONASAEROGINOSA OF MINKS
马俊 1张超 1曲新泽 1樊虓翀 1林逢生1
作者信息
- 1. 上海启盛生物科技有限公司，上海201203
- 折叠
摘要
Abstract
Total 425 isolates of suspected Pseudomonas aeroginosa were isolated from 2011 to 2013 from lungs, spleens and livers collected during breakouts of hemorrhagic pneumonia on mink farms in Shandong, Hebei and Jiangsu provinces. Two pairs of PCR primers were designed based on sequences of OprF and PEA genes, respectively. The DNA templates were extracted from these 425 isolates and amplified in PCR using the primer sets. The sizes of PCR products and nucleotide sequences confirmed that all 425 isolates were mink Pseudomonas aeroginosa. Serological typing divided these isolates into 4 serotypes:376 (88.5%) isolates in G serotype, 34 isolates (8.0%) in B serotype, 12 isolates (2.8%) in C serotype and 3 isolates (0.7%) in E serotype. Six isolates (3 from G serotype and one from each of other 3 serotypes) were randomly selected for further biochemical tests. All of these 6 isolates met requirements for Pseudomonas aeroginosa. In addition, 4 out of these 6 isolates (one serotype each) were inoculated into mice and minks and caused all animals sick.关键词
出血性肺炎/绿脓杆菌/血清型/分离与鉴定Key words
Hemorrhagic pneumonia/Pseudomonasaeroginosa/serotype/isolation and identification分类
农业科技引用本文复制引用
马俊,张超,曲新泽,樊虓翀,林逢生..水貂绿脓杆菌的分离与鉴定[J].中国动物传染病学报,2014,(1):44-49,6.
