中国动物传染病学报Issue(2)：1-6,6.
鸭坦布苏病毒E蛋白结构域III原核表达产物诱导中和抗体的研究
PROKARYOTIC EXPRESSION OF DOMAIN III OF ENVELOPE PROTEIN OF DUCK TEMBUSU VIRUS AND CHARACTERIZATION OF NEUTRALIZING ANTIBODY RESPONSES
摘要
Abstract
The domain III of envelope glycoprotein (E) of the flaviviruses has been shown to bind cellular receptors and elicit neutralizing antibodies. In the present study, recombinant domain III (DIII) of E protein of Duck Tembusu virus (DTMUV) was expressed in E.coli and its immunogenicity was evaluated using BALB/c mouse model. The coding sequence of DIII was optimized (optiDIII) according to the bias codon usages of E.coli and then cloned into the multiple cloning site of pCold-TF vector to construct the recombinant plasmid pCold-TF-optiDIII. Western blot analysis showed the recombinant protein could react specifically with DTMUV antiserum. The recombinant DIII was purified and used to immunize BALB/c mice three times. The humoral immune responses specific for DTMUV were demonstrated by ELISA and indirect immunofluorescene assay(IFA). Importantly, neutralizing antibodies against DTMUV were detected in mouse serum samples by microneutralizing test. These findings have indicated that the recombinant DIII can be used for development of diagnostic reagents and subunit vaccines of DTMUV.关键词
鸭坦布苏病毒/E蛋白/结构域III/中和抗体Key words
Duck Tembusu virus/envelope protein/domain III/neutralizing antibody分类
农业科技引用本文复制引用
余磊,李泽君,闫大为,高旭元,肖亚莉,刘绍琼,李雪松,闫丽萍,滕巧泱,李国新..鸭坦布苏病毒E蛋白结构域III原核表达产物诱导中和抗体的研究[J].中国动物传染病学报,2014,(2):1-6,6.基金项目
自然科学基金（面上）项目（31172332）；上海市科技兴农重点攻关项目（沪农科攻字（2012）第2-6号）；上海市科委“创新行动计划”基础研究重点项目（12JC1410600）；上海市科委“创新行动计划”现代农业领域重点科技攻关项目 （）
