中国动物传染病学报Issue(2)：32-38,7.
高致病性猪繁殖与呼吸综合征病毒SHxx13/2013株的分离与鉴定
CHARACTERIZATION OF RECENTLY ISOLATED SHXX13/2013 STRAIN OF HGHLY PATHOGENIC PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS
摘要
Abstract
In early 2013, an acute disease characterized by high fever and respiratory problems in piglets outbroke on a pig farm in Shanghai and caused many deaths. To investigate the causative agent, a total of 15 serum samples were collected and viruses were examined in RT-PCR. Eleven out of of 15 clinical samples showed strong Porcine reproductire and respiratory syndrone virus (PRRSV) signal and the same PCR fragments as compared with highly pathogenic PRRSV (HP-PRRSV). One PRRSV strain was isolated from a PCR positive sample and designated as SHxx13/2013 strain. The SHxx13/2013 strain induced cytopathic effect (CPE) on MarC-145 cells at the first passage, which shared similar plaque morphology and growth kinetics with HuN4 strain. The complete genome of SHxx13/2013 strain was 15 319 bp. The deletion of total 30 amino acids occurred at positions 482 and 534-562 in Nsp2 gene, which was a common feature of HP-PRRSV. The complete genome of the SHxx13/2013 strain was 99.6%identity to that of HuN4 strain, indicating that it was close to HuN4. The results concluded that HP-PRRSV was the causative agent in pig population in Shanghai.关键词
高致病性猪繁殖与呼吸综合征病毒/PCR/分离/鉴定Key words
Highly pathogenic Porcine reproductive and respiratory syndrome virus (HP-PRRSV)/PCR/isolation/identification分类
农业科技引用本文复制引用
程群,童光志,周艳君,姜一峰,虞凌雪,王康,杨莘,李丽薇,高飞,于海,童武..高致病性猪繁殖与呼吸综合征病毒SHxx13/2013株的分离与鉴定[J].中国动物传染病学报,2014,(2):32-38,7.基金项目
国家自然科学基金项目（31100121）；863计划项目（2011AA10A208-2）；上海市重点基础研究项目（11JC1415200）；国家生猪现代产业技术体系建设项目（NYCYTX-009）；上海市科技兴农重点攻关项目（沪农科攻字（2012）第2-5号） （）
