中国动物传染病学报Issue(2)：72-77,6.
柔嫩艾美耳球虫G6-PI基因的克隆、序列分析与原核表达
CLONING, SEQUENCE ANALYSIS AND PROKARYOTIC EXPRESSION OF GLUCOSE-6-PHOSPHATE ISOMERASE OF EIMERIATENELLA
摘要
Abstract
In order to analyze biochemical properties of Eimeria tenella glucose-6- phosphate isomerase EtG6-PI, the full-length of open reading frame Etg6-PI was amplified in RT-PCR from total RNA from the second-generation schizonts of E.tenella. The amplified fragments were Results showed that the open reading frame was 1650 bp, sequenced. The Etg6-PI gene was cloned into the expression vectors pColdI and pCold43a and expressed in E. coli Rosetta (DE3). encoded a protein of 550 amino acids. The recombinant vectors pColdI-EtG6-PI or pCold43a-EtG6-PI was transformed into E. coli Rosetta (DE3) cells and the expression was induced with IPTG Soluble protein obtained from pCold43a-EtG6-PI purifide by the affinity chromatography assay can be used for biochemical analysis of this enzyme. The prokaryotic expression of E.tenella G6-PI set important basis for function study on glucose-6-phosphate isomerase.关键词
柔嫩艾美耳球虫/葡萄糖-6-磷酸异构酶/克隆/原核表达Key words
Eimeriatenella/glucose-6-phosphate isomerase/cloning/prokaryotic expression分类
农业科技引用本文复制引用
刘田,廖申权,戚南山,吴彩艳,李娟,吕敏娜,李国清,孙铭飞..柔嫩艾美耳球虫G6-PI基因的克隆、序列分析与原核表达[J].中国动物传染病学报,2014,(2):72-77,6.基金项目
国家自然科学基金项目(31201902；31302087)；珠江科技新星项目(2012J2200059)；广东省重点实验室建设支撑项目（2012A061100006）；广东省对外科技合作项目 （31201902；31302087）
