中国实用口腔科杂志Issue(7)：417-420,4.
NF-κB/IκB信号通路对牙龈卟啉单胞菌感染诱导血管内皮细胞ICAM-1表达调控研究
Influence of nuclear factor-κB/inhibitoryκB pathway on intracellular adhesion molecule-1 expression induced by P.gingivalisin endothelial cells
摘要
Abstract
Objective To investigate whether intracellular adhesion molecule-1(ICAM-1)could be mediated by nucle-ar factor-κB(NF-κB)pathway in endothelial cells treated by P. gingivalis W83. Methods As we know production of ICAM-1 in endothelial cells could be increased by infection with P.gingivalis. Then the in vitro models of vascular endo-thelial cell infected with P. gingivalis W83 was established. Expression of ICAM-1 was examined by Real-time PCR and Western blotting in endothelial cells pretreated with P. gingivalis W83 for 8 h with or without the NF-κB antagonist MG132 at concentration of 5,10,20μmol/L respectively for 15,30,60 min. SAS 8.12 software was used for statistical anal-ysis;analysis of variance was done to compare the means. Results ICAM-1 production in endothelial cells increased by infection with P.gingivalis,MG132 could abrogate the production of ICAM-1 mRNA at concentration of 10μmol/L for 30 min,and the production of ICAM-1 was abrogated by MG132 at concentration of 20μmol/L for 15 min;in addition, the abrogation was more obvious at 30 and 60 min. Conclusion The induction of ICAM-1 by infection with P. gingiva-lis might be mediated by the NF-κB pathway in endothelial cells.关键词
牙龈卟啉单胞菌/核转录因子-κB/细胞间黏附分子-1Key words
P. gingivalis/NF-κB/ICAM-1分类
医药卫生引用本文复制引用
张冬梅,常春荣,潘亚萍..NF-κB/IκB信号通路对牙龈卟啉单胞菌感染诱导血管内皮细胞ICAM-1表达调控研究[J].中国实用口腔科杂志,2013,(7):417-420,4.基金项目
辽宁省博士科研启动基金（20121128）；人力资源和社会保障部留学人员项目 （）
