中国斜视与小儿眼科杂志Issue(1)：5-8,54,5.
反转缝合与丰富环境联合干预对成年弱视大鼠视皮层可塑性的再激活作用
Effect of reverse suture combined with enriched environment intervention on the reactivation of ocular dominance plasticity in the visual cortex of moncular form deprived adult rats
摘要
Abstract
Objective To investigate effect of reverse suture combined with enriched environment intervention on the reactivation of ocular dominance plasticity in the visual cortex of monocular form-deprived adult rats. Methods The 14-day-old rats were randomly divided into eight groups: 1~3 groups are normal control group (NC) , 4~8 groups are monocular deprivation group (MD) .The right eyelids of MD groups were sutured at postnatal 14 days to obtain animal model. Rats were housed in the standard environment. All the rats of 1~6 groups were sacrificed at postnatal 45, 60 and 90 days. The right eyelids of the rats for 7~8 groups were opened while left eyelids were sutured at postnatal 45 days.Rats of 7 group ( RS) were housed in the standard environment and rats of 8 group ( RS+EE) were housed in the enriched environment. The last two groups were sacrificed at postnatal 90 days. We measured the expression of PSD-95 protein in the visual cortex of the different groups by using immunohistochemistry staining. Results The density of positive neurons of PSD-95 in the visual cortex of MD group was significantly much less than those in NC groups at the same age ( P< 0.001) .The density of positive neurons of PSD-95 in RS group was higher than those of MD group (P< 0.05),but still lower than those of NC group at the same age (P< 0.05) . The density of positive neurons of PSD-95 in RS+EE group was higher than those of MD group and RS group at the same age ( P <0.05) , however, compared with NC group, the difference was not statistically significant ( P > 0.05) . Conclusions Monocular deprivation might influence the expression of PSD-95 in the visual cortex of monocular deprived form adult rats after the critical period, The results implied that PSD-95 might participated in the ocular dominance plasticity in the adult visual cortex. Effect of re verse suture combined with enriched environment intervention was much stronger than reverse suture alone intervention on the reactivation of ocular dominance plasticity in the visual cortex of monocular form deprived adult rats.关键词
反转缝合/弱视/单眼形觉剥夺/视皮层可塑性/PSD-95Key words
reverse suture/amblyopia/monocular form deprived/visual cortex plasticity/protein-95引用本文复制引用
王圆月,刘向玲,蔺静静,李靳,张佳娟,宋子宣,张锐..反转缝合与丰富环境联合干预对成年弱视大鼠视皮层可塑性的再激活作用[J].中国斜视与小儿眼科杂志,2014,(1):5-8,54,5.基金项目
新乡医学院2011年研究生科研创新支持计划资助项目(YJSCX 201123Y) （YJSCX 201123Y）
