中国烟草科学Issue(5)：78-82,5.DOI:10.3969/j.issn.1007-5119.2013.05.016
烟草CHS基因RNA干扰载体的构建及其遗传转化
Construction of RNAi Vectors of CHS Gene and its Transformation in Tobacco
摘要
Abstract
Flavonoid are important aroma precursors, which are controlled by many regulatory genes. Chalcone synthase gene (CHS), as one of the key gene of the flavonoid pathway, plays an important role in the synthesis of aroma precursors. The function missing or mutation of the CHS would affect the aroma formation. To explore the effects of CHS down regulatory expression and to provide technical information for breeding high aroma tobacco, we constructed the RNAi expression vectors by Gateway technology. Then the vectors were transformed into the‘Dabaijin599’ by Agrobacterium-mediated method to form basis for further study in the aroma of CHS gene. Thirty of tobacco plants were obtained and 24 of them were identified as transgenic plants.关键词
烟草/查尔酮合成酶/CHS/基因/RNAi载体/遗传转化Key words
tobacco/Chalcone synthase/CHS/gene/RNAi vectors/transformation分类
农业科技引用本文复制引用
李尊强,杨爱国,常爱霞,张长静,郑吉云,冯全福..烟草CHS基因RNA干扰载体的构建及其遗传转化[J].中国烟草科学,2013,(5):78-82,5.基金项目
国家烟草专卖局科技重点项目“优质抗病烤烟新品种选育及育种技术研究” （）
