中国烟草科学Issue(6)：103-107,5.DOI:10.3969/j.issn.1007-5119.2013.06.020
陈化烟叶中纤维素降解菌群的分离及其特性研究
Isolation and Characterization of Bacterial Community with Cellulose-Decomposition Ability from Aging Flue-cured Tobacco Leaves
摘要
Abstract
Large amounts of bacteria have been found on aging flue-cured tobacco leaves. A bacterial community with the ability to decompose cellulose was isolated from aging flue-cured tobacco leaves. The effects of temperature, pH value and different cellulose source on the cellulase activity of bacterial community were further investigated. 16S rDNA PCR-DGGE technology was used to analyze bacterial community composition. The results showed that the bacterial community mainly consisted of five species. At 20℃, the cellulase activity of the bacterial community was very low. With the temperature increasing, the cellulase activity increased, and reached the peak at 60℃. When the temperature exceeded 60℃, the cellulase activity then decreased dramatically. With the pH value increasing from 3 to 8, the cellulase activity increased gradually, and reached the peak at pH 8. When the pH value increased from 8 to 9, the cellulose activity dropped sharply. The bacterial community was able to decompose the cellulose in the tobacco leaves, filter paper and the tobacco stalk. The cellulase activity of the bacterial community fermented in the tobacco leaves was significantly higher than that in the filter paper and tobacco stalk. In conclusion, the bacteria may have influence on the cellulose decomposition of tobacco leaves and affect the character of the tobacco during aging. The bacterial community may be used to decompose the cellulose of tobacco leaves and tobacco stalk in order to improve the quality of the tobacco leaves and stalk.关键词
陈化烟叶/纤维素降解/菌群/纤维素酶活性Key words
aging flue-cured tobacco leaf/cellulose decomposition/bacterial community/cellulose activity分类
农业科技引用本文复制引用
关亮,李天丽,陈竹亭,周亚维,汤朝起,瞿永生,李朋富..陈化烟叶中纤维素降解菌群的分离及其特性研究[J].中国烟草科学,2013,(6):103-107,5.基金项目
上海烟草集团有限责任公司烟草行业卷烟烟气重点实验室开放课题（00554）；江苏省自然科学基金 （）
