中国烟草科学Issue(1)：37-42,6.DOI:10.13496/j.issn.1007-5119.2014.01.007
谷氨酰胺合成酶抑制剂对衰老期烟叶氮代谢的影响
The Influence of Glutamine Synthetase Inhibitors on Nitrogen Metabolism of Tobacco Leaves during Senescence Period
摘要
Abstract
By using the method of apoplast extraction and determination of enzymes related nitrogen metabolism, the influence of Glufosinate (GS) inhibitor concentrations on nitrogen metabolism physiological indices of flue-cured tobacco varieties with two nitrogen use efficiency were studied. The results show that GS inhibitors could inhibit GS activity of tobacco leaf during senescence, reduce the ability of nitrogen assimilation, promote the degradation of nitrogen nutrient, induct the increasing of glutamate dehydrogenase (GDH) activity and decreasing of nitrate reductase (NR) activity and promote accumulation of leaf NH4+, increase ammonia volatilization potential. These inhibition effects became more obvious with increasing GS inhibitor concentration. Compared with high nitrogen efficiency varieties, low nitrogen efficiency varieties aged more rapidly and had a lower NH4+accumulation and ammonia volatilization potential. There was significant difference between varieties. This shows that GS inhibitors can accelerate leaf senescence and promote nitrogen degradation and transfer.关键词
烤烟/氮代谢/氮利用率/谷氨酰胺合成酶抑制剂Key words
flue-cured tobacco/nitrogen metabolism/physiological N utilization efficiency/glutamine synthesis inhibitors分类
农业科技引用本文复制引用
武云杰,杨铁钊,张小全..谷氨酰胺合成酶抑制剂对衰老期烟叶氮代谢的影响[J].中国烟草科学,2014,(1):37-42,6.基金项目
中国烟草总公司重大专项（Ts-01-2011003）；中国烟草总公司项目 （）
