中国烟草学报Issue(5)：112-117,6.DOI:10.3969/j.issn.1004-5708.2013.05.020
防治烟草黑胫病有益内生细菌的筛选及其抑菌作用
Screening of endophytic beneficial bacteria to tobacco black shank control and its bacteriostasis
摘要
Abstract
127 endophytic bacteria strains were isolated from roots, stems and leaves of healthy tobacco plant by the method of grinding separation in different growing stages, and their antagonistic activities to Phytophthora parasitica var.nicotianae were studied to find new endophytic bacteria for biological control. Results showed that 12 strains were antagonistic to growth of P. parasitica var.nicotianae with width of inhibitory zone above 5mm. Those isolates were assayed for antagonistic activity, fermentation activity in vitro and greenhouse experiment. Results indicated that there existed active ingredients in cell-free culture of 8 bacteria strains, among which wy2, wy11 and wy4 had the greatest antibacterial activity against P. parasitica var nicotianae with inhibition rate above 30%. Their filtrates could damage the hyphal structure of P. parasitica var. nicotianae, resulting in abnormal expansion of hyphae damage of cell wall, exosmosis of cytoplasm, collapse of hyphae cells, and slow growth of bacteria. Results of greenhouse experiments showed that strain wy11 was the most effective strain against P. parasitica var nicotianae with control effect reached 51.7%. In addition, bacterial suspensions of endophytic bacteria wy11 and cj17 had certain growth-promoting effects on tobacco plants, which could increase stem girth, leaf length and leaf width by irrigating roots. Phylogentic analysis based on 16S rDNA suggested that wy11 shared 99%homologies with B. amyloliquefaciens (AB255669). These sequences constituted a branch in phylogenetic tree. Strain wy11 was identified as Bacillus amyloliquefaciens.关键词
烟草黑胫病菌/内生细菌/生物防治/促生作用Key words
Phytophthora parasitica var.nicotianae/endophytic bacteria/biological control分类
农业科技引用本文复制引用
陈泽斌,夏振远,雷丽萍,陈海如..防治烟草黑胫病有益内生细菌的筛选及其抑菌作用[J].中国烟草学报,2013,(5):112-117,6.基金项目
云南省烟草公司科技项目（No.2012YN05）；国家自然科学基金资助项目(No.30860017) （No.30860017）
