中国烟草学报Issue(1)：26-31,6.DOI:10.3969/j.issn.1004-5708.2014.01.005
苯酚提取-超高效液相色谱法测定烟草中的磷酸腺苷
Determination of adenosine phosphate in tobacco leaf by UPLC with phenol-TEA pretreatment
摘要
Abstract
A analytical method for simultaneous determination of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in fresh tobacco leaves was established by ultra high performance liquid chromatography (UPLC) with phenol extraction. ATP, ADP and AMP in phenol extraction were stable and difficult to be hydrolyzed with rate of change less than 10%within 2 hours. A better separation of ATP, ADP and AMP was achieved in 10 minutes by utilizing a gradient elution system of 6‰triethylamine (pH=6.6) and methanol as mobile phase. The linear range was between 5~200 µg.mL-1 with adenosine concentration and peak area were highly correlated (r>0.9999). The detection limits for ATP, ADP and AMP were 0.5 µg.mL-1, 0.4 µg.mL-1, 0.3 µg.mL-1 respectively, and average recovery rate was 91.68%~110.79%with RSD ranged between 1.53%~3.67%. This method can quickly and accurately detect ATP content in tobacco samples, thus providing important test indicators for the study of energy metabolism, especially tobacco CMS generation mechanism.关键词
超高效液相色谱/磷酸腺苷/苯酚/新鲜烟草Key words
UPLC/adenosine phosphate/phenol/fresh tobacco leaves分类
轻工纺织引用本文复制引用
林小峰,吴玉萍,陈学军,孔光辉..苯酚提取-超高效液相色谱法测定烟草中的磷酸腺苷[J].中国烟草学报,2014,(1):26-31,6.基金项目
国家烟草专卖局“烟草育种新技术研究及新一轮优质抗病烤烟新品种选育” （）
