中国烟草学报Issue(1)：96-102,7.DOI:10.3969/j.issn.1004-5708.2014.01.018
DNA烷基化损伤及其修复研究进展
Research advances in DNA alkylation damage and repair
田永峰 1侯宏卫 2张小涛 3刘勇 1胡清源 2王安1
作者信息
- 1. 中国科学院安徽光学精密机械研究所，合肥 230031
- 2. 国家烟草质量监督检验中心，郑州高新区枫杨街2号 450001
- 3. 国家烟草质量监督检验中心，郑州高新区枫杨街2号 450001
- 折叠
摘要
Abstract
The fact that DNA alkylation damage might lead to mismatch in replication was considered as cause of gene mutation and other relevant disease. The variety of DNA alkylation damage, repair mechanisms and its products,the generative mechanism in vivo and analytical method of alkylation DNA adducts were reviewed. Current analytical methods of alkylation DNA adducts include Immunoaffinity Chromatography/32P-postlabelling technique (IC-32P), Gas Chromatogeraph-Mass Spectrometer (GC-MS) and Liquid Chromatography-tandem Mass Spectrometer (LC-MS/MS). IC-32P had excellent performance on sensibility, but it took many complicated steps in determining alkylation DNA adducts. The sample usually cost more in the method of GC-MS during pre-handling process as derivatization is needed;LC-MS/MS method offered many practical advantages in pre-handling such as stability, selectivity and sensibility, which can enhance the efficiency of sample analysis. As biomarkers of DNA alkylation damage, alkylation DNA adducts played a significant role in risk evaluation of alkylation reagents.关键词
DNA烷基化损伤/烷基化DNA加合物/DNA烷基化损伤修复Key words
DNA alkylation damage/DNA adducts/repair of DNA alkylation damage分类
轻工纺织引用本文复制引用
田永峰,侯宏卫,张小涛,刘勇,胡清源,王安..DNA烷基化损伤及其修复研究进展[J].中国烟草学报,2014,(1):96-102,7.
