中国药理学通报Issue(4)：506-509,510,5.DOI:10.3969/j.issn.1001-1978.2014.04.014
神经生长因子对H9 c2心肌细胞缺氧/复氧后凋亡的保护作用及其机制
Anti-apoptotic effect of NGF on H9 c2 cardiac myocytes in a hypoxia/reoxygenation injury model
摘要
Abstract
Aim To investigate the anti-apoptotic effect of NGF on H9 c2 cardiac myocytes in a hypoxia / reox-ygenation injury model and its mechanism. Methods The H9 c2 cardiac myocytes were randomly divided into five groups:control group ( C group) , hypoxia/reoxy-genation group ( H/R group) , NGF group ( N group) , NGF+LY294002 group ( N+L group) and LY294002 group( L group) . Each group received the correspond-ing treatment. Cell survival rate was tested by cell counter kit-8 methods. Apoptotic rate was evaluated by propidium iodide ( PI ) staining and flow cytometry (FCM). The levels of Caspase-12, p-Akt/Akt were e-valuated by Western blot. Results The NGF group could significantly protect the H9 c2 cardiac myocytes under the hypoxia / reoxygenation injury with increased cell survival rate. It also decreased the apoptotic per-centage, upregulated the level of p-Akt/Akt and inhib-ited the expression of Caspase-12 . As the specific in-hibitor of PI3k receptor, LY294002 decreased the level of p-Akt. Conclusion NGF has the effect of anti-ap-optosis on H9 c2 cardiac myocytes exposed to hypoxia /reoxygenation injury via PI3k-Akt signal pathway.关键词
H9 c2 心肌细胞/缺氧/复氧损伤/神经生长因子/PI3k-Akt通路/心肌细胞存活率/心肌细胞凋亡率/内质网应激蛋白Key words
H9 c2 cardiac myocytes/hypoxia / reoxy-genation injury/nerve growth factor/PI3 k-Akt signal pathway/survival rate of cardiac myocytes/apoptotic percentage/endoplasmic reticulum stress protein分类
医药卫生引用本文复制引用
谢飞,魏珂,闵苏,郝学超,朱贤林..神经生长因子对H9 c2心肌细胞缺氧/复氧后凋亡的保护作用及其机制[J].中国药理学通报,2014,(4):506-509,510,5.基金项目
国家自然科学基金青年基金项目( No 81000066) （ No 81000066）
卫生部国家临床重点专科[ No 财社〔2011〕170号] （）
重庆市医学重点学科建设项目[ No 渝卫科教〔2007〕2号] （）
