中国药理学通报Issue(4)：536-541,6.DOI:10.3969/j.issn.1001-1978.2014.04.021
灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌AGEs及CTGF的影响及其机制
Effect of combination of ganoderma lucidum polysaccharide and metformin on AGEs and CTGF of cardiac muscle in type 2 diabetic rats and the mechanism
摘要
Abstract
Aim To study the effects of ganoderma lu-cidum polysaccharides and metformin on myocardial fi-brosis of type 2 diabetic rats and its mechanism. Methods SD rats were fed with high fat diet for 4 weeks, and then were injected with streptozotocin (30mg·kg-1 ) to replicate type 2 diabetic model. The diabetic rats were randomized into normal control group,diabetes group, ganoderma lucidum polysaccha-rides group ( 600 mg · kg-1 ) , metformin group ( 600 mg·kg-1 ) , and combination group( ganoderma lucid-um polysaccharides 300 mg·kg-1 +metformin 300 mg ·kg-1 ) . After 12 weeks’ treatment,the levels of fast-ing serum glucose were determined and the extent of myocardial fibrosis was observed by Picro-sirius red staining. The contents of AGEs in serum were deter-mined by fluorescence spectrophotometer. The activities of CAT and GSH-Px in myocardium were detected. Im-munohistochemical method and Western blot were used to detect myocardial tissue AGEs and CTGF protein ex-pression. Results Combination group could repress patho-proceeding of myocardial fibrosis efficiently, im-prove the activity of CAT and GSH-Px in myocardium and lower the concentration of AGEs in serum, as well as reduce the expression of AGEs and CTGF in myo-cardium. Conclusions Ganoderma lucidum polysac-charides and metformin could prevent myocardial fibro-sis. The possible mechanism may be related to repress-ing oxidative stress of myocardium, lowering serum AGEs and down regulating AGEs and CTGF of myocar-dium.关键词
灵芝多糖/二甲双胍/糖尿病大鼠/心肌纤维化/晚期糖基化终末产物/结缔组织生长因子/氧化应激Key words
ganoderma lucidum polyccharide/met-formin/diabetes mellitus rats/myocardial fibrosis/AGEs/CTGF/oxidative stress分类
医药卫生引用本文复制引用
乔进,窦志华,吴锋,孟国梁,陈惠,郑惠华..灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌AGEs及CTGF的影响及其机制[J].中国药理学通报,2014,(4):536-541,6.基金项目
科技部“十一五”国家科技支撑计划子课题项目( No 2006DAI06A20-02) （ No 2006DAI06A20-02）
江苏省优势学科项目 （）
