中国药理学通报Issue(4)：554-558,5.DOI:10.3969/j.issn.1001-1978.2014.04.025
卤米松、黄芩提取物联合用药对白癜风小鼠模型的影响
Effect of halometasone in combination with scutellaria baicalensis georgi on the vitiligo mice
摘要
Abstract
Aim To study the effect of halometasone in combination with scutellaria baicalensis georgi on the vitiligo mice induced by monobenzone. Methods 40% monobenzone cream was applied to induce vitiligo in C57BL/6 mice. Through the halometasone, halo-metasone and scutellaria baicalensis georgi combined with 40% monobenzone cream, the influence of halo-metasone and scutellaria baicalensis georgi on mice de-colorizing was studied. Hair decolorizing was observed with the naked eye, the skin decolorizing was observed by reflectance confocal microscopy ( RCM ) , and CD8 +T cell infiltration was tested with immunofluores-cence detection. The serum levels of interleukin-6(IL-6 ) and tumor necrosis factor-α( TNF-α) were deter-mined by enzyme linked immunosorbent assay ( ELISA) . Results Mice in model group showed de-pigmentation at both the monobenzone application part and non-application part. The halometasone group did not show significant therapeutic efficacy. In halometa-sone and scutellaria baicalensis georgi treatment group, there was less decolorization, the occurrence ratio, the scores of occurring time and size were lower compared with model group. There were fewer infiltrated lympho-cytes and CD8 +T cells. Halometasone and scutellaria baicalensis georgi group also showed that the serum levels of IL-6,TNF-α decreased. Conclusion Halo-metasone and scutellaria baicalensis georgi have thera-peutic effect on vitiligo mice induced by monobenzone.关键词
卤米松/黄芩/白癜风/动物模型/CD8 +T细胞/IL-6/TNF-αKey words
halometasone/scutellaria baicalensis geor-gi/vitiligo/models animal/CD8 +T cell/IL-6/TNF-α分类
医药卫生引用本文复制引用
祝逸平,王遂泉,卢良君,许爱娥..卤米松、黄芩提取物联合用药对白癜风小鼠模型的影响[J].中国药理学通报,2014,(4):554-558,5.基金项目
国家自然科学基金资助项目( No 81071294) （ No 81071294）
省部共建项目( No WKJ2012-2-036) （ No WKJ2012-2-036）
市科委重大专项创新项目( No 20122513A02) （ No 20122513A02）
浙江省自然科学基金项目( No LY13H11001) （ No LY13H11001）
省科技厅项目(No 2011C23134) （No 2011C23134）
国家临床重点专科建设项目 （）
