中国药理学与毒理学杂志Issue(2)：199-204,6.DOI:10.3867/j.issn.1000-3002.2014.02.009
焦炉逸散物致支气管上皮细胞 Th17细胞因子改变
Changes of Th17 cytokine in human bronchial epithelial cells induced by coke oven emission
摘要
Abstract
OBJECTIVE To find the infla mmation bio markers induced by coke oven e missions (COE),we investigated the changes of T helper 17 (Th17 )cytokines in hu man bronchial epithelial (16HBE)cells.METHODS 16HBE cells were exposed to organic extracts of COE collected fro m co-king plant at the concentrations of 5,10 and 20 mg·L -1 for 24 h or 5 d to establish short-term and long-term cell models,respectively.Cell viability was measured by MTT assay and infla mmatory da mage was assessed by lactate dehydrogenase assay (LDH).The cytokines in culture supernatant sa mples was detected by co mmercial hu man Th17 cytokine panel kit.RESULTS COE Can induce infla mmation in COE 20 mg·L -1 group and no expression on IL-17 F and IL-1 β.The concentration of IL-10 was 1 .25 ± 0.54,1 .39 ±0.13 and (1 .90 ±0.73)pg·mL -1 in COE 5,10 and 20 mg·L -1 group showing good con-centration-effect relationship (r=0.98,P <0.05 ).IL-23 expression was found only higher at 10 and 20 mg·L -1 and the concentrations were 3.38 ±3.90 and (1 .74 ±2.00 )pg·mL -1 ,respectively.In 16HBE cells treated by COE for 5 d,elevated expression of IL-17A was found in COE 5 and 10 mg·L -1 group,and there was statistically sigificant difference between COE 10 mg·L -1 and DMSO group (P<0.05).Elevated concentration of IL-17F of 10.2 ±1 1 .78 and (6.79 ±7.84)pg·mL -1 was found in COE 5 and 10 mg·L -1 group.The concentration of IL-10 was 1 .71 ±0.02,1 .49 ±0.25 and (2.82 ± 0.33)pg·mL -1 in COE 5,10 and 20 mg·L -1 group,respectively.We found increased IL-1 βexpression with concentration of 2.72 ±0.62,2.25 ±0.33 and (0.93 ±0.21 )pg·mL -1 in COE 5,10 and 20 mg·L -1 group with negative dose-response relationship.We also found more elevated TNF-αlevels in the 5 d than in the 24 h model with no COE specific relationship.CONCLUSION COE induces expression changes of Th17 cytokines profile in 16HBE cells,including IL-23 and IL-1 βfor early and long-term infla mmation,respectively.IL-10 may be a candidate marker for population study on COE induced infla mmatory injury.关键词
焦炉逸散物/支气管上皮细胞/乳酸脱氢酶/Th17 细胞/细胞因子Key words
coke oven e missions/bronchial epithelial cells/lactate dehydrogenase/Th17/cytokines分类
资源环境引用本文复制引用
李红丽,解秋艳,刘秀玲,牛勇,戴宇飞,郑玉新,姚林,段化伟..焦炉逸散物致支气管上皮细胞 Th17细胞因子改变[J].中国药理学与毒理学杂志,2014,(2):199-204,6.基金项目
国家自然科学基金（81172642）；国家自然科学基金（81130050）；国家高技术研究发展计划（863计划，2012AA062804）；国家科技支撑计划（2014BAI12B02）@@@@The project supported by the National Natural Science Foundation of China （81172642）；National Natural Science Foundation of China （81130050）；National High-Tech Research and Develop ment Progra m of China （2012AA062804）；and National Key Technology Research and Develop ment Progra m （）
