中国药理学与毒理学杂志Issue(2)：262-266,5.DOI:10.3867/j.issn.1000-3002.2014.02.020
双复磷对沙林染毒所致兔眼缩瞳及视觉障碍的对抗作用
Antagonism of obidoxime on sarin induced miosis and visual impairment in rabbits
摘要
Abstract
OBJECTIVE The antagonism of obidoxi me on sarin induced miosis and visual impair-ment was evaluated and its antagonistic mechanism was investigated.METHODS ① 30 min after sarin (2 μg /0.1 mL per eye)was given as an eyedrop,the ability of the 2.5%,5.0%,7.5% obidoxi me and 1 .0% atropine to reverse effects of sarin on pupil dia meter and light reflex were evaluated at different ti mes.② Another 36 rabbits received sarin and at 30 min afer sarin exposure,the drugs above were ad-ministrated and their effects on pupillary light reflex,as well as the AChE activity of cornea,iris and reti-na were recorded 4h after the treatment.RESULTS ① Miosis and impaired pupillary light reflex oc-curred soon after sarin exposure but the abnormal pupil width and pupillary light reflex had disappeared by 48 h after sarin exposure;Subcequent to 1 .0% atropine treatment,the pupil dilatedinstead while the impaired light reflex did not i mprove significantly;unlike atropine,soon after ad ministration of 2.5%, 5.0%,7.5% obidoxi me,the pupil dia meter and light reflex were significantly increased(P <0.01 )and then had beco me normal totally by 24 h post-dose,much faster than those of the control and atropine treatment group.However,there was no significant difference in the recovery ti me between the different dose groups of obidoxi me.② 4h after treatment,the AChE activity in cornea and irisof sarin-treated group were (42 ±4)%,(26 ±2)%,respectively;the AChE activity in cornea of 2.5%,5.0%,7.5%obidoxi me were (74 ±1 1 )%,(81 ±10)% and (74 ±7)%,respectively,and the AChE activity in iris were(39 ±10)%,(43 ±8)% and (43 ±8)%,respectively ,co mpared with sarin-treated group,AChE activities of cornea and iris as well as light reflex of the obidoxi me-treated group were significantly increased(P<0.01 ).But there was no difference in light reflex and AChE activity between the sarin-treated and atropine-treated groups.CONCLUSION Obidoxi me showed better antagonism of sarin-induced ocular effects than that of the commonly used drug,atropine;the antagonistic mechanism is likely closely related to its rapid reactivation of the inhibited AChE in the cornea and iris.关键词
沙林/缩瞳/对光反射调节力/双复磷/乙酰胆碱酯酶Key words
sarin/miosis/light reflex regulation/obidoxi me/acetylcholineaterase分类
医药卫生引用本文复制引用
程凤,李万华,骆媛,杨军,聂志勇,隋昕,刘艳芹,薛艳萍,王永安..双复磷对沙林染毒所致兔眼缩瞳及视觉障碍的对抗作用[J].中国药理学与毒理学杂志,2014,(2):262-266,5.基金项目
国家科技重大专项（2011 ZXJ09102-05C）；国家科技重大专项（2011 ZXJ09305）；全军医学科技“十二五”重大项目（AWS11 C004）；全军医学科技“十二五”重点项目（BWS024）@@@@ The project supported by National Major Special project of Drug Develop ment of China （2011 ZXJ09102-05C）；National Major Special project of Drug Develop ment of China（2011 ZXJ09305）；The Twelfth Five Year Major Special Project of Medicine Research of PLA（AWS11 C004）；The Twelfth Five Year Mega-Special Project of PLA（BWS12J042）；and The Youth Cultivation Foundation of the General Logistics Depart ment of PLA （）
