中国药理学与毒理学杂志Issue(2)：267-273,7.DOI:10.3867/j.issn.1000-3002.2014.02.021
麝香酮对斑马鱼胚胎的发育毒性
Developmental toxicity of muscone on zebrafish embryos
摘要
Abstract
OBJECTIVE To investigate the develop mental toxicity of muscone to embryos. METHODS With zebrafish embryos as a model,The 3 h post fertilization (hpf)embryos were exposed to muscone at 5,10,20,40,80 and 160 μmol·L -1 culture solutions for 96 h and inspected daily with mi-croscopy for larval morphology.The drug solution was replaced every 24 h.Spontaneous move ments were checked at 24 hpf.Heart rate at 48 hpf,hatching rate,e mbryo deformity rate and mortality rate were evaluated.The expression of sepn1 was determined with real-ti me quantitative PCR technique at 96 hpf.RESULTS The 24 hpf spontaneous move ments showed no significant difference.At 48 hpf, spine curvature,pericardial ede ma,yolk sac ede ma,and abnormal swi mming were observed.In addition, the 48 hpf heart beats(10 s)was decreased fro m 26.5 ±1 .0 to 18.0 ±1 .9(P <0.01 ).At 48 hpf , hatching rate of 5 ~40 μmol·L -1 decreased(P <0.05),while of 160 μmol·L -1 increased (P <0.05) co mpared with muscone 0 μmol·L -1 .Muscone had little effect on hatching rate at other ti me points;Mal-formation rate and mortality rate at higher concentrations were up to 100%.The sepn1 gene expression at 96 hpf in the exposure groups decreased co mpared with that of control group(P <0.01 ).CONCLU-SION Muscone had toxic effects on the develop ment of zebrafish embryos,including spine curvature, abnormal swi mming,and pericardial ede ma.These effects may be related to the inhibition of sepn1 gene expression by muscone.关键词
麝香/麝香酮/斑马鱼/胚胎发育/婴幼儿用药Key words
moschus/muscone/zebrafish/e mbryo develop ment/infant medication分类
医药卫生引用本文复制引用
陈怡君,钟玉绪,董武,李春杰,王丽星,蒲韵竹,尚艳楠,赵宝全,刘萍..麝香酮对斑马鱼胚胎的发育毒性[J].中国药理学与毒理学杂志,2014,(2):267-273,7.基金项目
国家自然科学基金（81173572）@@@@The project supported by National Natural Science Foundation of China （）
