中国医学创新Issue(36)：10-12,3.DOI:10.3969/j.issn.1674-4985.2013.36.004
β-细辛醚对抑郁模型大鼠海马CA3区超微结构的改变及脑组织ERK蛋白表达的影响
Effect of Beta-asarone in Hippocampus CA3 Region and the Expression of Protein ERK In Rat Model of Depression
摘要
Abstract
Objective:To observe the effect of beta-asarone on CA3 ultrastructure in hippocampus and the expression of ERK protein in the hippocampus of depression model rats. Method:4-5 month old 60 male Sprague-Dawley rats were randomly divided into normal control group,model control group,β-asarone group,fluoxetine control group,each group contained 15 rats. 21 d accepted chronic unpredictable mild stress. From the second day,Western medicine and Chinese medicine were given fluoxetine at the same time andβ-asarone intervention. On the 1,7,14,21th day,open-field experiments,detection of sugar consumption and body weight were detected respectively in rats as assessed by indicators such as detection of behavior change. Change of hippocampus was observed by electron microscope. Protein ERK expression in brain tissue was assayed by immunohistochemical test and computer image analysis technology. Result:Number of apoptotic cells of hippocampal CA3 area in model control group were found increased,andβ-asarone could alleviate the damage of hippocampus neurons. Compared with the model control group,the protein expressions of ERK in the hippocampus inβ-asarone group and fluoxetine group increased with statistical significance(P<0.05). Conclusion:Beta-asarone can obviously improve the depressive state of the model rats. Its mechanism might be related with increasing the protein expressions ERK and reducing the brain neural cell apoptosis.关键词
β-细辛醚/抑郁/ERK/免疫组织化学染色/电镜Key words
βeta-asarone/Depression/ERK/Immunohistochemical test/Computer image analysis technology引用本文复制引用
荣华,董海影,张静艳,张晓杰..β-细辛醚对抑郁模型大鼠海马CA3区超微结构的改变及脑组织ERK蛋白表达的影响[J].中国医学创新,2013,(36):10-12,3.基金项目
国家自然科学基金 （）
