中国医学创新Issue(3)：1-2,3,3.DOI:10.3969/j.issn.1674-4985.2014.03.001
肝动脉灌注化疗抑制肝癌血管生成的研究
The Research of Hepatic Arterial Infusion Chemotherapy in Inhibiting Angiogenesis in Hepatocellular Carcinoma
摘要
Abstract
Objective:To study the effects on the angiogenesis in hepatocellular carcinoma of rats through hepatic arterial infusion chemotherapy. Method:After the rat hepatocellular carcinoma model was established,18 rats with two-week intrahepatic inoculated tumor were randomly divided into the control group,the peripheral vein group and the hepatic artery group,and six rats in each group.The 5-FU dosage of rat was 20 mg/kg and the rats were administered continuously for 4 days.The liver tumors were removed at the seventh day after the end of chemotherapy,and the expression of VEGF protein and CD34 protein in HCC were detected by Immunohistochemical methods.The value of integrated optical density(IOD)was analyzed by VEGF stained protein,and the tumor microvessel density(MVD)was detected by the marker vascular endothelial cells of CD34.Result:The expression of VEGF(IOD value)in the control group was(46351.48±2926.24),it was significantly higher than that of peripheral vein group(43678.84±2852.76) and hepatic artery group(35518.86±2657.16),and the expression of VEF in hepatic artery group was significantly lower than that of peripheral vein group,the differences were statistically significant(P<0.05).CD34 protein and MVD expression in liver tissue of the hepatic artery group was the lowest (36.26±8.43),it was significantly lower than that of peripheral vein group(62.08±10.77)and the control group(69.74±16.60),the differences were statistically significant(P<0.05).CD34 protein and MVD expression in liver tissue between the peripheral vein group and the control group were not statistically significant(P>0.05).Conclusion:Hepatic arterial infusion chemotherapy can effectively inhibit the expression of VEGF and reduce the MVD in HCC tissues,so as to inhibit the angiogenesis of hepatocellular carcinoma.关键词
肝癌/肝动脉灌注化疗/血管生成/血管内皮生长因子/微血管密度Key words
Hepatocellular carcinoma/Hepatic arterial infusion chemotherapy/Angiogenesis/VEGF/MVD引用本文复制引用
郑惊雷,梁力建,王在国,陈刘镇,胡夏荣,叶振伟..肝动脉灌注化疗抑制肝癌血管生成的研究[J].中国医学创新,2014,(3):1-2,3,3.基金项目
广东省医学科研基金（B2010324），东莞市科技计划医疗卫生项目 （）
