中国医学创新Issue(11)：1-2,3,3.DOI:10.3969/j.issn.1674-4985.2014.11.001
甲状腺乳头状癌miR-34b基因表达及其启动子区甲基化的临床意义研究
The Clinical Significance of miR-34b Gene Expression and Its Promoter Region Methylation in Papillary Thyroid Carcinoma
董子龙 1张生彬 1武妍婷1
作者信息
- 1. 内蒙古医科大学第三附属医院 内蒙古 包头 014010
- 折叠
摘要
Abstract
Objective:To discuss the clinical significance of miR-34b gene expression and its promoter region methylation(MSP)in papillary thyroid carcinoma(PTC). Method:From October 2008 to January 2013,carcinoma tissue specimens(the control group)in 42 patients with papillary thyroid carcinoma and pericarcinomatous tissue specimens (the experimental group)in 42 patients with thyroid adenoma were collected,then miR-34b mRNA expression(real time quantitative PCR method)and miR-34b gene promoter region methylation(methylation-specific PCR method)of two groups were detected and analyzed.Result:In experimental group,the average expression level of miR-34b mRNA was(0.84±0.04), the positive rate of miR-34b gene promoter methylation was 71.4%. Compared with the control group,there were statistically significant difference(P<0.05). In the experimental group,the methylation rate were no significant difference between different gender,age,tumor diameter,stage,and capsular invasion(P>0.05). However,the rates of lymph node metastasis methylation was(88.5%),it was significantly higher than that of no lymph node metastasis(43.8%),there was a statistically significant difference(P<0.05).Conclusion:There is a close relationship between miR-34b gene expression and promoter region methylation development,a deep clinical study is recommended.关键词
甲状腺乳头状癌/miR-34b基因/甲基化Key words
Papillary thyroid carcinoma/miR-34b gene/Methylation引用本文复制引用
董子龙,张生彬,武妍婷..甲状腺乳头状癌miR-34b基因表达及其启动子区甲基化的临床意义研究[J].中国医学创新,2014,(11):1-2,3,3.
