摘要
Abstract
Objective:To investigate the influence of propofol with different concentrations on the expression of Bcl-2 and Bax in Wistar rat model of hepatic carcinoma. Method:50 Wistar rats were divided into 5 groups according to random number table method,each group had 10 rats,the normal group(the group A),the liver cancer model group(the group B), the low dose Propofol group:3 mg/kg(the group C1),the moderate dose Propofol group:6 mg/kg(the group C2),the high dose Propofol group:12 mg/kg(the group C3). The liver tissue were collected in 24 h after the last injection and observed by morphology and hematoxylin and eosin(HE)staining. S-P immunohistochemistry was used to examine the expression of Bcl-2 and Bax in liver tissue of different groups of Wistar rats. Result:(1)Morphology and HE staining:compared with the group A,the group became B ragged and yellow spots appeared on some surfaces. Liver cells appeared steatosis,spotty necrosis and inflammatory cell infiltration by HE staining. Compared with the group B,there were less steatosis and spotty necrosis in the group C(C1,C2,C3).(2)TUNEL staining showed that increased the dosage of Propofol,apoptosis cells of liver cancer cells increased,the AI was increased gradually,the difference was statistical significance(P<0.05).(3) Compared with the group A,the expression of Bcl-2 increased,and the expression of Bax decreased in the group B,the difference was statistically significant(P<0.05). Compared with the group B,the Bcl-2 expression decreased liver cells in the group C(C1,C2 and C3),and increased with the increase of dosage of Propofol showed a trend of decline,the difference was statistically significant(P<0.05);Bax expression was raised,and increased with the increase of Propofol dose increased trend,the difference was statistically significant(P<0.05). Conclusion:The rate of Bcl-2/Bax is decreased by Propofol, which could inhibit the proliferation of HCC cells.关键词
丙泊酚/肝癌/细胞凋亡/Bcl-2/BaxKey words
Propofol/Hepatic carcinoma/Apoptosis/Bcl-2/Bax
