中国癌症防治杂志Issue(4)：291-295,5.DOI:10.3969/j.issn.1674-5671.2013.04.01
绿色荧光蛋白标记的鼻咽癌放射抗拒性裸鼠移植瘤模型的建立及其活体成像观察
Establishment and dynamic in vivo imaging of a radioresistant,GFP-labelled xenograft tumor model of human nasopharyngeal carcinoma in mouse
摘要
Abstract
Objective To establish a GFP-labelled radioresistant xenograft tumor model of human nasopharyngeal carcinoma(NPC) and build the foundation for further study. Methods Lentivirus was used to establish a stable,radioresistant human NPC cell line expressing GFP(GFP-CNE-2R)and a corresponding non-radioresistant cell line(GFP-CNE-2).Both lines were subcutaneously implanted into BALB/c-nu nude mice;when the tumors had reached 1 cm in size,the animals were irradiated with 10 Gy in 1 fraction.Tumor proliferation and metastasis were analyzed in vivo using dynamic imaging technology. Results GFP-CNE-2R and GFP-CNE-2 lines were successfully established,and clone formation assays showed GFP-CNE-2R cells to be radioresistant.Xenograft tumors were grown from GFP-CNE-2R and GFP-CNE-2 cells,and growth of GFP-CNE-2R tumors was not significantly inhibited by radiation. Conclusion GFP-CNE-2R xenograft tumors are more radioresistant than GFP-CNE-2 xenograft tumors.关键词
鼻咽肿瘤/移植瘤/绿色荧光蛋白/放射抗拒性Key words
Nasopharyngeal neoplasms/Xenograft tumor/Green fluorescent protein/Radiation resistance分类
医药卫生引用本文复制引用
黄小鹏,马慧,朱小东,葛莲英,曲颂,李龄,郭亚,赵伟,黎丹戎..绿色荧光蛋白标记的鼻咽癌放射抗拒性裸鼠移植瘤模型的建立及其活体成像观察[J].中国癌症防治杂志,2013,(4):291-295,5.基金项目
国家自然科学基金资助项目（30860329）；广西高等学校重大科研资助项目（201101D004）广西医疗卫生重点科研项目 （）
