中国医药科学Issue(17)：30-32,3.
熊果酸对HL-60细胞株凋亡及Bcl-2和bax表达的影响
Effect of ursolic acid on apoptosis and expressions of Bcl-2/bax in HL-60 cells
摘要
Abstract
Objective To investigate the effect of UA on the apoptosis and expressions of Bcl-2/bax in HL-60 cells. Methods HL-60 cell line was cultured in vitro. MTT methods was used to detect the inhibitory effect of UA on proliferation. Flow cyetmetry was used to observe the effect of UA on the apoptosis and cycle distribution of HL-60 cells. The influence of UA on the bcl-2 and bax protein expression of HL-60 cells was analyzed with western blot. Results UA markedly inhibited proliferation of HL-60 cells in a concentration-dependent manner, UA induced apoptosis of HL-60 cells in a concentration dependent manner,and decreased the proportion of cells in the G1 phase. UA can efficiently up-regulated bax and down-regulated bcl-2 protein expression of HL-60 cells in a concentration dependent manner. Conclusion UA inhibited proliferation and induced apoptosis on HL-60 cells partly by up-regulating expression of bax and down-regulating expression of bcl-2.关键词
人急性髓性白血病/熊果酸/凋亡/Bcl-2Key words
Human acute myeloid leukaemia/Chrysin/Apoptosis/Bcl-2分类
医药卫生引用本文复制引用
岳军..熊果酸对HL-60细胞株凋亡及Bcl-2和bax表达的影响[J].中国医药科学,2013,(17):30-32,3.基金项目
湖南省教育厅青年项目资助（10B099）。 （）
