中国医药科学Issue(18)：9-10,41,3.
目视LAMP技术对人乳头瘤病毒18亚型检测的应用价值研究
Application value study of visual loop-mediated isothermal amplification in human papillomavirus type 18 diagnosis
摘要
Abstract
Objective To investigate the application of loop-mediated isothermal amplification(LAMP)in detecting Human papillomavirus type 18(HPV18) from clinical specimens. Methods It is characterized by employing a set of four specially designed primers spanning six distinct sequences of a target gene HPV18-L1. The reaction was performed at 65℃for 1 hour with the addition of calcein dye prior to amplification. LAMP was used for sensitivity assay and detecting HPV18 mono infected in cervical secretions from women of physical examination and patients. Results The sensitivity of LAMP for detection of HPV18 L1 is 10-3 per microliter;the positive rate of HPV18 in patients with vaginitis is 5.26%(1/19); the positive rate of HPV18 in patients with chronic cervicitis is 10%(2/20); and the positive rate of HPV18 in patients with cervical intraepithelial neoplasia (CIN I-Ⅱ)and cervical cancer-I is 33.3%(3/9), 64%(16/25), respectively. Conclusion LAMP assay is a high efficiency, low cost diagnostic tool, useful for rapid, accurate, direct detection of HPV for clinical diagnosis and might be popular at the primary hospital in future.关键词
人乳头瘤病毒/核酸/环介导等温扩增试验Key words
Human papillomavirus/Nucleotide acids/Loop-mediated isothermal amplification分类
医药卫生引用本文复制引用
唐宏霞,熊学峰,秦志强,周晓农..目视LAMP技术对人乳头瘤病毒18亚型检测的应用价值研究[J].中国医药科学,2013,(18):9-10,41,3.基金项目
国家科技重大专项课题（2012ZX10004-220）。 （）
