中国中医药信息杂志Issue(7)：52-54,3.DOI:10.3969/j.issn.1005-5304.2013.07.019
HPLC-ELSD测定养血益肾胶囊中黄芪甲苷的含量
Determination of Astragaloside IV in Yangxue Yishen Capsules by HPLC-ELSD
彭娟 1闫寒 1郭娜 1聂颖兰 1范斌1
作者信息
- 1. 中国中医科学院医学实验中心，北京 100700
- 折叠
摘要
Abstract
Objective To establish the quality standard for Yangxue Yishen Capsules. Methods Rhizoma Gastrodiae, Herba Epimedii and Radix et Rhizoma Salviae Miltiorrhizae in the formula were identified qualitatively by TLC. The content of Astragaloside IV was determined by HPLC-ELSD method. The Diamonsil C18(2) column (4.6 mm×250 mm, 5 µm) was used. The mobile phase was acetonitrile-water (33∶67) with 1.0 mL/min flow rate at 35 ℃. The temperature of drift tube for ELSD was 105 ℃, the air flow rate was 2.7 L/min. Results The TLC displayed clear spots on their right positions repeatedly. The linear range of Astragaloside IV was 0.397 0-5.955 0 µg (r=0.999 9). The average recovery was 102.0%, RSD was 1.5% (n=6). Conclusion The method is simple and quick. It can be used as quality control method of Yangxue Yishen Capsules.关键词
养血益肾胶囊/黄芪甲苷/薄层色谱法/高效液相色谱-蒸发光散射检测器Key words
Yangxue Yishen Capsules/Astragaloside IV/TLC/HPLC-ELSD分类
医药卫生引用本文复制引用
彭娟,闫寒,郭娜,聂颖兰,范斌..HPLC-ELSD测定养血益肾胶囊中黄芪甲苷的含量[J].中国中医药信息杂志,2013,(7):52-54,3.
