中国中医药信息杂志Issue(12)：51-53,3.DOI:10.3969/j.issn.1005-5304.2013.12.019
HPLC测定马钱子及其提取物中马钱子碱和士的宁含量
Determination of Brucine and Strychnine in Semen Strychni and Its Extractive by HPLC
摘要
Abstract
Objective To develop a RP-HPLC method for the determination of Brucine and Strychnine in Semen Strychni and its extractive of total alkaloids. Methods A chromatographic column of Licrospher C18 (4.6 mm×250 mm, 5 μm) was used with the mobile phase of acetonitrile∶0.01 mol/L sodium heptane sulfonate and 0.02 mol/L potassium dihydrogen phosphate mixed with equal amount (adjusted pH to 2.8 with 10% phosphonic acid)=27∶73, detection wavelength at 260 nm, column temperature of 30 ℃ and flow rate of 1 mL/min. Results The calibration curves of Brucine and Strychnine were both in good linearity in the ranges of 0.1-1.0 µg and 0.12-1.2 µg (r=1.000) respectively. The average recovery rates of Brucine and Strychnine were 99.88% (RSD=1.06%) and 100.06% (RSD=0.78%) respectively. Conclusion The method is realiable and accurate, which can be applied to determination of Brucine and Strychnine in Semen Strychi and its extractive.关键词
马钱子/马钱子碱/士的宁/含量测定/高效液相色谱法Key words
Semen Strychni/Brucine/Strychnine/determination/HPLC分类
医药卫生引用本文复制引用
刁国兰,张贵华,马云淑,张文平,黄金娥,罗红梅..HPLC测定马钱子及其提取物中马钱子碱和士的宁含量[J].中国中医药信息杂志,2013,(12):51-53,3.基金项目
国家自然科学基金(30960517) （30960517）
