中国中医药信息杂志Issue(1)：50-53,4.DOI:10.3969/j.issn.1005-5304.2014.01.016
不同工艺提取的竹节参皂苷粗提物毒性及其抗炎作用初步研究
Toxicity and Anti-inflammatory Effect of Total Saponins from Panax japonicus by Different Extraction Technology
摘要
Abstract
Objective To compare the toxicity and anti-inflammatory effect of total saponins from Panax japonicus by different extraction technology. Methods The total saponins of sample 1, sample 2, sample 3, sample 4, sample 5 and sample 6 was prepared respectively by different process, and RAW264.7 cells were treated with the samples of different concentration. Then cells morphology was observed under microscope, thiazolyl blue (MTT) method was used to detect cell activity, the nitric oxide (NO) release of RAW264.7 cells was detected with NO kit. Results The cell toxicity of different samples from low to high was as follows:sample 4<sample 6<sample 5<sample 2<sample 1<sample 3. The NO inhibitory effect on RAW264.7 cells stimulated by LPS was as follows:sample 4>sample 5>sample 2>sample 6>sample 1>sample 3. Conclusion Among these six different kinds of extraction process of total saponins from Panax japonicus, the total saponins extract by foam fractionation has not only the minimal toxicity, but also the best primary anti-inflammatory effect.关键词
皂苷/提取工艺/竹节参皂苷粗提物/抗炎/细胞活力Key words
saponins/extraction process/saponins from Panax japonicus/anti-inflammatory/cell activity分类
医药卫生引用本文复制引用
代艳文,袁丁,张海滨,万静枝,孙志伟,陈赤清,张长城,王婷..不同工艺提取的竹节参皂苷粗提物毒性及其抗炎作用初步研究[J].中国中医药信息杂志,2014,(1):50-53,4.基金项目
国家自然科学基金(31070314、81374001)；高等学校青年教师深入企业行动计划项目(XD2012122) （31070314、81374001）
