中国中医药信息杂志Issue(3)：75-77,78,4.DOI:10.3969/j.issn.1005-5304.2014.03.022
清肾颗粒质量标准研究
Study on Quality Standard of Qingshen Granules
摘要
Abstract
Objective To establish the quality standard of Qingshen Granules. Methods Thin-layer chrmatography was used to identify Rheum officinale Baill., Hedyotis Diffusa Wild. and Coptis chinensis Franch.. The contents of rhein, emodin and chrysophanol were determined by HPLC. The HPLC consisted of Kromasil C18 (4.6 mm×250 mm, 5 μm), methanol-0.1% phosphonic acid (70∶30) as mobile phase, flow rate of 1.0 mL/min, detection wavelength at 245 nm, and the column temperature was 30 ℃. Results The results of TLC showed that relevant spots were clear without interference against the negative sample. The calibration curves for rhein, emodin and chrysophanol were found to be linear within the range of 0.003 3-0.42 μg (r =0.999 9), 0.008 0-0.51 μg (r=1.000 0), 0.009 9-0.32 μg (r=1.000 0), respectively. The average recoveries were 98.84%, 99.04% and 100.35% with RSD of 1.30% (n=6), 1.34% (n=6) and 1.89% (n=6), respectively. Conclusion The methods are accurate and quick in qualitative identification and quantitative assay, and can be used for the quality control of Qingshen Granules.关键词
清肾颗粒/质量标准/薄层色谱法/高效液相色谱法Key words
Qingshen Granules/quality standard/TLC/HPLC分类
医药卫生引用本文复制引用
石金敏,高家荣,李娆娆,贾陆,顾雪竹..清肾颗粒质量标准研究[J].中国中医药信息杂志,2014,(3):75-77,78,4.基金项目
北京市自然科学基金面上项目(7112097)；中国中医科学院基本科研业务费自主选题项目(ZZ2006096、2011xycz-12) （7112097）
