中国中医药信息杂志Issue(4)：55-58,4.DOI:10.3969/j.issn.1005-5304.2014.04.018
舒心稳斑颗粒对动脉粥样硬化兔血管内皮生长因子和基质金属蛋白酶-9表达的影响
Effects of Shuxinwenban Granules on Expression of VEGF and MMP-9 in Rabbits
摘要
Abstract
Objective To observe the anti-atherosclerosis (AS) mechanisms of Shuxinwenban granules by observing its effects on the serum level of MMP-9, the expression of VEGF and the formation of the plaques in rabbits. Methods Totally 40 healthy female and male rabbits were divided into control group, model group, Shuxinwenban granules group and Simvastatin group. Control group was treated with basic diet, and other groups were treated with high cholesterol diet for 12 weeks to make AS rabbit models. The treatment groups were given corresponding drugs by gavage for 4 weeks. The serum levels of MMP-9 in rabbits were detected by double antibody sandwich enzyme-linked immunosorbent method and the surface density of VEGF in the atherosclerotic plaques by immunohistochemical staining, at the same time, the pathological changes of the aorta and atheroclerotic plaques were observed. Results Compared with the model group, Shuxinwenban granules significantly decreased the serum levels of MMP-9, and the difference was statistically significant (P <0.01). Similarly, Shuxinwenban granules could significantly decrease the surface density of VEGF in atherosclerotic plaques, the difference was also statistically significant (P<0.01). Pathology showed that the intervention of Shuxinwenban granules decreased the area of atherosclerotic plaque, caused the plaque to smaller and thinner. Conclusion Shuxinwenban granules blocked the inflammatory response by inhibiting the expression of VEGF in atherosclerotic plaque and decreasing the levels of MMP-9 in serum, which lessened the formation of the atherosclerotic plaques.关键词
舒心稳斑颗粒/动脉粥样硬化斑块/血管内皮生长因子/基质金属蛋白酶-9/兔Key words
Shuxinwenban granules/atherosclerotic plaques/VEGF/MMP-9/rabbits分类
医药卫生引用本文复制引用
黎鹏程,卢丽丽,尹勇,聂娅..舒心稳斑颗粒对动脉粥样硬化兔血管内皮生长因子和基质金属蛋白酶-9表达的影响[J].中国中医药信息杂志,2014,(4):55-58,4.基金项目
湖南省中医药管理局课题(2010011)；湖南中医药大学重点学科-中医基础理论(2007年) （2010011）
