中国中医药信息杂志Issue(4)：86-88,89,4.DOI:10.3969/j.issn.1005-5304.2014.04.027
川芎药材HPLC指纹图谱研究
Study on Fingerprint of Chuanxiong Rhizoma by HPLC
摘要
Abstract
Objective To establish analysis methods for fingerprint of Chuanxiong Rhizoma by HPLC. Methods Thermo C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used with methanol-0.1% Formic acid in gradient elution. The flow rate was 1 mL/min, the detection wavelength was set at 323 nm, and the temperature was 25 ℃. The similarities of the 18 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of 18 batches of Chuanxiong Rhizoma, 11 common peaks were identified, the similarities were almost greater than 0.9 among all batches. The samples were clustered into 3 categories. Conclusion The method is simple, steady and repeatable. It provides a basis for the quality control and evaluation of Chuanxiong Rhizoma.关键词
川芎/指纹图谱/高效液相色谱法Key words
Chuanxiong Rhizoma/fingerprint/HPLC分类
医药卫生引用本文复制引用
贡磊,狄留庆,陈凌云,李杰,严燕清,李全..川芎药材HPLC指纹图谱研究[J].中国中医药信息杂志,2014,(4):86-88,89,4.基金项目
江苏省科技成果转化资金项目(BA2012078)；江苏省产学研前瞻性项目(BY2013008-01) （BA2012078）
