中华耳科学杂志Issue(4)：602-606,5.DOI:10.3969/j.issn.1672-2922.2013.04.034
小鼠鼓室注射小干扰RNA导入内耳可行性研究
Delivery of Short Interfering RNA Through Otic Bone Into Mouse Cochlea
摘要
Abstract
Objective To investigate the advantages of the method of intra-tympanic injection through the otic bone for the delivery of siRNA into the adult mouse cochlea. Methods A fluorescently-conjugated scrambled siRNA probe was ap-plied via intra-tympanic injection into the middle ear cavity to detect if the siRNA probe could be delivered into organ of corti and HSP70 siRNA was delivered into cochlea to reduce the expression of HSP70 in hair cells. Results Fluorescently-conjugat-ed scrambled siRNA probe was detected in sensory hair cells and nerve fibers 3 days after the injection. Intra-tympanic deliv-ery of HSP70 siRNA successfully reduced immunofluorescence associated with HSP70 in outer hair cells 3 days after injection. Conclusion Intra-tympanic injection through the otic bone into the middle ear cavity provides a promising approach for deliv-ery of compounds or siRNA to cochlear hair cells of mice, relevant for the study of mechanisms underlying inner ear insults.关键词
小干扰RNA/小鼠/毛细胞Key words
siRNA/Mice/hair cells分类
医药卫生引用本文复制引用
郑宏伟,韩琳,余力生..小鼠鼓室注射小干扰RNA导入内耳可行性研究[J].中华耳科学杂志,2013,(4):602-606,5.
